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Melting-temperature profiles of five different HBV strains. The amplicon generated by the HBV DNA real-time NASBA was located between nucleotides 613 and 817 (see arrow), which is situated in a part of the HBV genome that has a relatively low melting temperature. A low template melting temperature theoretically facilitates the annealing of primers and probes in a NASBA or PCR. The box at the right shows serotype, GenBank accession number, and genotype information for the isolates.
