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Comparison of effect of different extraction methods on detection of HBV DNA by NASBA. (A) Protocol Y, standard silica-guanidiniumthiocyanate extraction method; (B) protocol Y+, addition of α-casein to lysis buffer; (C) protocol H, proteinase K digestion prior to extraction; (D) protocol H+, both proteinase K digestion prior to extraction and addition of α-casein to lysis buffer.
