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Comparison of the pKa values determined on the basis of the thermophoresis/TRIC at different heating times (25 s and 2.5 s), and the reference values obtained by methods using fluorescence but independent of the thermophoresis/TRIC (MST and CE-LIF). The error of pKa determination is given in parentheses. The same temperature (22 °C) was set up in the MST and CE-LIF instruments.
