TABLE 1.
Detection of DEN-1 in C6/36 cells infected at different MOIs on day 1 postinoculation using indirect IF stain
| MAb | Virus strain | No. of positive cells detected at an MOI of:
|
|||
|---|---|---|---|---|---|
| 0.1 | 0.01 | 0.001 | 0.0001 | ||
| 15F3-1 (anti-NS1) | Hawaii | <1a (2+)b | —c | — | — |
| 766733 | <1 (2+) | — | — | — | |
| 066267 | <1 (2+) | — | — | — | |
| 8-8 (anti-E) | Hawaii | 4 (2+) | <1 (1+) | — | — |
| 766733 | 78 (3+) | 5 (1+) | — | — | |
| 066267 | 80 (3+) | 6 (1+) | — | — | |
| 16-4 (anti-NS1) | Hawaii | 12 (4+) | 5 (1+) | <1 (1+) | +/—d |
| 766733 | 78 (3+) | 5 (1+) | <1 (1+) | +/— | |
| 066267 | 80 (3+) | 7 (1+) | <1 (1+) | +/— | |
a
Mean number of dengue virus-positive cells under observation in four different fields. Magnification, ×400.
b
Numbers in parentheses represent a rating of fluorescence intensity assigned arbitrarily.
c
—, negative result.
d
+/−, equivocal result.