TABLE 2.
Comparison of three fixation-permeabilization methods for the detection of dengue virus in C6/36 cells using flow cytometrya
| Fixation-permeabilization method | Mean percentage of positive cellsb
|
||||
|---|---|---|---|---|---|
| DEN-1 MAb (15F3-1) | DEN-1 MAb (16-4) | DEN-2 MAb (3451) | DEN-3 MAb (504-11) | DEN-4 MAb (IH10-6) | |
| Paraformaldehyde-Tween-20 | 49.45 | 54.40 | 88.92 | 71.30 | 66.72 |
| Paraformaldehyde-methanol | 56.78 | 70.68 | 47.70 | 88.99 | 78.72 |
| Paraformaldehyde-Triton X-100 | 67.43 | 72.36 | 86.92 | 93.65 | 95.10 |
a
C6/36 cells were infected with DEN-1 (Hawaii), DEN-2 (New Guinea C), DEN-3 (H87) and DEN-4 (H241) at an MOI of 0.1 and incubated at 28°C for 7 days.
b
Mean percentage of positive cells by two measurements.