TABLE 4.
Comparison of the sensitivity in detection of DEN-1 virus (Hawaii)-infected C6/36 cells by conventional IF staining and flow cytometrya
| Virus inoculation (PFU) | Results of detection of dengue virus antigen in C6/36 cells at 3 days postinoculation
|
|||
|---|---|---|---|---|
| IF staining
|
Flow cytometryb
|
|||
| 15F3-1 | 16-4 | 15F3-1 | 16-4 | |
| 1,000 | + | + | + (1.16) | + (3.23) |
| 100 | + | + | + (0.98) | + (2.40) |
| 10 | − | + | + (0.88) | + (1.87) |
| 1 | − | − | + (0.37) | + (0.96) |
| 0 | − | − | − (≤0.1) | − (≦0.1) |
a
C6/36 cells were infected with DEN-1 (Hawaii) in 6-well plates and incubated at 28°C for 3 days.
b
Percentages in parentheses represent mean percentages of positive cells reacted with MAb 16-4 by two measurements.