Table 4.
Circular dichroism-derived protein secondary structure composition of hemp seed protein isolate (HPI) and fractions (2S, 7S, and 11S) at different pH values.
| pH | Samples | α-Helix (%) | β-Sheet (%) | β-Turns (%) | Unordered (%) |
|---|---|---|---|---|---|
| pH 3 | 2S | 80.40 ± 0.00 | 0.70 ±0.00 | 5.95 ± 0.01 | 1.53 ± 0.03 |
| 7S | 4.40 ± 0.00 | 31.18 ± 0.00 | 17.30 ± 0.00 | 46.66 ± 0.00 | |
| 11S | 3.10 ± 0.01 | 37.45 ± 0.02 | 17.45 ± 0.01 | 43.65 ± 0.00 | |
| HPI | 2.20 ± 0.01 | 43.45 ± 0.03 | 19.45 ± 0.01 | 34.95 ± 0.00 | |
| pH 5 | 2S | 18.60 ± 0.00 | 3.90 ± 0.01 | 12.50 ± 0.03 | 65.05 ± 0.04 |
| 7S | 1.40 ± 0.00 | 39.50 ± 0.00 | 20.35 ± 0.00 | 39.05 ± 0.00 | |
| 11S | 1.55 ± 0.00 | 43.25 ± 0.02 | 21.30 ± 0.01 | 35.50 ± 0.00 | |
| HPI | 1.85 ± 0.00 | 41.50 ± 0.01 | 20.30 ± 0.00 | 36.35 ± 0.01 | |
| pH 7 | 2S | 1.35 ± 0.01 | 37.55 ± 0.02 | 17.55 ± 0.00 | 47.05 ± 0.01 |
| 7S | 2.20 ± 0.00 | 40.80 ± 0.02 | 19.05 ± 0.03 | 38.85 ± 0.03 | |
| 11S | 3.45 ± 0.00 | 44.25 ± 0.00 | 20.30 ± 0.00 | 32.20 ± 0.00 | |
| HPI | 17.40 ± 0.01 | 26.05 ± 0.01 | 20.20 ± 0.04 | 36.15 ± 0.04 | |
| pH 9 | 2S | 4.55 ± 0.02 | 34.30 ± 0.00 | 17.30 ± 0.00 | 46.90 ± 0.00 |
| 7S | 3.30 ± 0.00 | 37.10 ± 0.00 | 17.05 ± 0.00 | 46.60 ± 0.00 | |
| 11S | 0.00 ± 0.00 | 55.45 ± 0.02 | 23.60 ± 0.01 | 24.15 ± 0.00 | |
| HPI | 0.00 ± 0.00 | 45.95 ± 0.01 | 13.70 ± 0.00 | 39.99 ± 0.03 |