FIGURE 2.

BLG in bovine urine samples, revealed by anti‐bovine BLG ELISA (A–F), silver stain (G), and immunoblot (H). BLG content of urine samples of cattle (n = 91; undiluted) from different stables determined by ELISA. Statistical comparison between (A) sexes among all animals, (B) sexes between adult animals, (C) lactation status of all animals, (D) season of urine sample collection (n = 57), (E) age of all animals, and (F) feeding conditions of beef cattle and dairy cattle (n = 57). Individual results are dotted on logarithmic scale on y‐axis, median is indicated. Indication: **p < 0.01, ****p < 0.0001; no indication: not significantly different. Presence of BLG was confirmed in (G) silver staining and (H) BLG‐specific immunoblot in selected urine samples (BLG values from ELISA indicated in ng/ml). BLG: commercial BLG at 1 and 0.1 μg/lane