Fig. 2.
BH4 increases Bortezomib resistance in MM. a MPC-11 and MOPC-315 cells were cultured in the presence of BH4, Bortezomib (Bor), or their combinations (BH4 + Bor) for 24 h. Cells treated with PBS served as controls. Cell apoptosis was analyzed by flow cytometry. Numbers in the dot plots represent viability of MM cells. Showing are representative data of three independent experiments. Right, summarized results of three independent experiments obtained as left. Data are presented as mean ± SD. b, c Balb/c mice were injected s.c. with MPC-11 (b) or MOPC-315 (c) cells (1 × 106 per mouse). From day 6 after tumor challenge, mice were injected i.p. with Bor or BH4 + Bor every 2 days. Mice received PBS served as controls. Shown are the tumor growth curves. The experiments were performed twice with a total of 10 mice per group (n = 10). Data are presented as mean ± SD. *P < 0.05; **P < 0.01