Fig. 4.

BH4 increases P53 degradation and NF-κB activation via USP7 and USP46. a MPC-11 were cultured with or without the additions of BH4, Bor, or their combinations (BH4 + Bor) for 2 h. Western-blots examined the protein levels of pIkBα, IkBα, and p53. β-actin was used as a loading control. The samples derive from the same experiment and that gels/blots were processed in parallel. b MPC-11 were cultured with or without the additions of BH4, Bor, or their combinations (BH4 + Bor) for 5 h. The nuclear protein levels of P50 and P65 were examined by western-blots. Histone H3 was used as a loading control. c MPC-11 were cultured with USPi, Bor, BH4 + Bor, or BH4 + Bor + USPi for 2 h. Western-blots examined the protein levels of pIkBα, IkBα, and p53. The samples derive from the same experiment and that gels/blots were processed in parallel. d MPC-11 were cultured with USPi, Bor, BH4 + Bor, or BH4 + Bor + USPi for 5 h. The nuclear protein levels of P50 and P65 were examined by western-blots. Data are representative (a–d) of three independent experiments