Fig. 3.

Growth under high or low iron conditions and siderophore production measured by CAS assay of parental strain (A) V. anguillarum RV22 ∆vabF, its derivative ∆frpA mutant (B) (RV22 ∆vabF∆frpA), and control strain (C) (RV22 ∆vabD) that is unable to synthetize any siderophore. The frpA complemented strain (D) was obtained by the introduction of the wild-type frpA gene, cloned in the plasmid pSEVA, into V. anguillarum ∆frpA mutant