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Inhibition of the mTOR pathway inhibits viral replication and its activation enhances it. Rapamycin attenuated viral replication as shown by reduced copies of SARS-CoV-2 N1 and N2 copies (A,B) and Spike protein; pooled data in (C) and representative image in (D). (A–D; n = 3 biological replicates). Analysis was done using One Way ANOVA with Tukey's test for multiple comparisons: *p < 0.05, **p < 0.01, ***p < 0.001 using a 95% CI. Scale Bar: 200 μm.
