FIGURE 2.

High MCAM expression was associated with poor OS prognosis through promoting tumor cell migration. (A) MCAM expression was significantly higher in human OS tissue samples compared to normal tissue samples (GSE16088, n = 14 and 6, respectively). (B) MCAM expression was significantly higher in the human OS tissues compared to primary non‐neoplastic osteoblast cells (GSE14359, n = 18 and 2, respectively). (C) MCAM expression was significantly higher in osteosarcoma stem cells (OSC, n = 4) and adherent osteosarcoma cells (AOC, n = 4) compared with mesenchymal stem cells (MSC, n = 4) (GSE52063). (D) Network of the top 10 genes correlated with MCAM. (E) GO analyses in Metascape for the top 10 genes that correlated with MCAM. (F) GO analyses in Metascape for the top 100 co‐expressed genes with MCAM. (G) MCAM expression in OS patients with and without metastasis (GSE21257, n = 34 and 19, respectively). Unpaired two‐tailed t test was used for the analysis