FIGURE 4.

Bradykinin receptors (BRs) expression in the nodose ganglia (NG) and nucleus tractus solitary (NTS) in male, age‐matched female, and ovariectomized (OVX) rats. The protein expression and distribution were assessed by western blot and immunostaining with specific antibody against B1R or B2R. White and orange arrowheads: HCN1‐positive (myelinated A‐ and Ah‐types) and HCN1‐negative (unmyelinated C‐types) neurons in the NG; (A‐B) the protein expression and distribution of B1R in the NG and NTS tissues; (C‐D) the protein expression and distribution of B2R in the NG and NTS tissues; Averaged data were presented as mean ± SD; *p < 0.05 and **p < 0.01 vs. male; ^# p < 0.05 and ^## p < 0.01 vs. female (mRNA: n = 4–5 from 4–5 rats, protein: n = 3–6 from 6–12 rats)