TABLE 1.
Biochemical and cultural characteristics and drug susceptibility testing results for various subspecies of M. tuberculosis complexa
| Subspecies (no. of strains) | Colony morphology | Biochemical test results
|
Growth in the presence of:
|
Drug susceptibility
|
CLR MIC (μg/ml) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Niacin production | Presence of:
|
Tween 80 hydrolysis | Presence of β-glucosidase | TCH | NAP | TB10 | SM | d-CS | Other drugs | PZA | |||||||
| Nitrate reductase | Catalase
|
Urease | Aryl sulfatase | ||||||||||||||
| 22°C | 68°C | ||||||||||||||||
| M. tuberculosis (50) | Eugonic (rough) | + | + | + | − | + | − | + | + | R | S | S | S | S | S | S | 20 |
| M. bovis BCG (10) | Eugonic (rough) | − | − | + | − | + | − | − | − | S | S | S | S | R | S | R | 0.5 |
| M. bovis (20) | Dysgonic (rough) | − | − | + | − | − | − | − | − | S | S | S | S | S | S | R | 10 |
| M. africanum (15) | Dysgonic (rough) | X | X | + | − | X | − | X | X | S | S | S | S | S | S | S | 10 |
| M. canettii CIPT140010059 | Eugonic (smooth) | − | + | + | − | + | − | + | − | R | S | S | R | S | S | R | >32 |
| M. canettii 9600046 | Eugonic (smooth) | − | + | + | − | + | − | + | − | R | S | S | R | S | S | R | >32 |
| M. canettii 910563 | Eugonic (smooth) | − | + | + | − | + | − | + | − | R | S | S | R | S | S | R | >32 |
| M. canettii 9701549 | Eugonic (smooth and rough) | − | + | + | − | + | − | + | − | R | S | S | R | S | S | R | >32 |
| M. canettii 217/94 | Eugonic (smooth) | − | + | + | − | + | − | + | − | R | S | S | ND | S | S | R | >32 |
| M. canetti 17727 (So93R) | Eugonic (rough) | − | + | + | − | + | − | + | − | R | S | S | R | S | S | R | ND |
| M. canetti 17728 (So93) | Eugonic (smooth) | − | + | + | − | + | − | + | − | R | S | S | R | S | S | R | >32 |
Symbols and abbreviations: +, positive result; −, negative result; X, variable results; R, drug resistant; S, drug suseptible; TCH, thiophene-2-carboxylic acid hydrazide (5 μg/ml); NAP, p-nitro-α-acetylamino-β-hydroxypropiophenone (5 μg/ml); TB10, thiacetazone (10 μg/ml); PZA, pyrazinamide (50 μg/ml); SM, streptomycin (2 μg/ml); d-CS, d-cycloserine (30 μg/ml); CLR, clarithromycin; ND, not done. Other drugs included isoniazid (0.2 μg/ml), rifampin (1 μg/ml), ofloxacin (1.5 μg/ml), kanamycin (6 μg/ml), amikacin (4 μg/ml), clofazimine (1 μg/ml), rifabutin (1 μg/ml), ciprofloxacin (1.5 μg/ml), and ethambutol (2 μg/ml). The Arylsulfatase result was read at day 3, whereas the Tween 80 hydrolysis result was read at day 10; Drug susceptibility and growth experiments were performed with 7H11 agar medium except for tests for growth in the presence of TB10 and susceptibility to d-cycloserine and ethambutol, which were performed on Löwenstein-Jensen medium.