TABLE 1.
Figure | Panel | Condition | Age | Comparison to Control | Test | p-value | Control Animal N | Experimental Animal N |
---|---|---|---|---|---|---|---|---|
2 | E | norm area POD Crestin + stain | 32 hpf | vp16-hoxb5b | T-test | 7.75E-07 | 27 | 21 |
2 | E | norm area POD Crestin + stain | 50 hpf | vp16-hoxb5b | T-test | 1.14E-03 | 7 | 8 |
3 | E | norm area POD foxd3 + stain | 32 hpf | vp16-hoxb5b | Welch’s T-test | 5.71E-03 | 7 | 10 |
3 | F | norm area hindbrain Phox2bb + stain | 32 hpf | vp16-hoxb5b | Welch’s T-test | 0.01 | 7 | 8 |
3 | G | norm area POD phox2bb + stain | 32 hpf | vp16-hoxb5b | Welch’s T-test | 5.63E-03 | 7 | 8 |
3 | O | POD phox2b + cells | 32 hpf | 15 pg vp16-hoxb5b | T-test | 3.03E-05 | 7 | 9 |
3 | O | POD phox2b + cells | 32 hpf | 30 pg vp16-hoxb5b | T-test | 2.61E-05 | 7 | 5 |
4 | I | norm area POD Crestin + stain | HS 14|24 hpf | hsp70:GFP-hoxb5b+ | T-test | 0.02 | 6 | 8 |
4 | J | norm area POD Crestin + stain | HS 22|26-28 hpf | hsp70:GFP-hoxb5b+ | T-test | 0.04 | 10 | 14 |
5 | E | # total Enteric Phox2b + cells | 50 hpf | 15 pg vp16-hoxb5b | T-test | 1.83E-04 | 10 | 7 |
5 | E | # total Enteric Phox2b + cells | 50 hpf | 30 pg vp16-hoxb5b | Welch's T-test | 3.01E-04 | 10 | 9 |
5 | F | # POD phox2b + cells | 50 hpf | 15 pg vp16-hoxb5b | Wilcoxon Test | 3.28E-03 | 10 | 7 |
5 | F | # POD phox2b + cells | 50 hpf | 30 pg vp16-hoxb5b | Kruskal-Wallis Test | 6.80E-04 | 10 | 9 |
5 | J | # Total Kaede + cells | 3 dpf | 15 pg vp16-hoxb5b | T-test | 1.10E-04 | 7 | 8 |
5 | J | # Total Kaede + cells | 3 dpf | 30 pg vp16-hoxb5b | T-test | 4.09E-05 | 7 | 7 |
5 | K | # Total Elavl3/4 + cells | 3 dpf | 15 pg vp16-hoxb5b | T-test | 1.35E-03 | 7 | 8 |
5 | K | # Total Elavl3/4 + cells | 3 dpf | 30 pg vp16-hoxb5b | T-test | 1.04E-04 | 7 | 7 |
5 | L | Copositive Elavl3/4+/Kaede + cells of Total Kaede + cells | 3 dpf | 15 pg vp16-hoxb5b | Wilcoxon Test | 0.13 | 7 | 8 |
5 | L | Copositive Elavl3/4 +/Kaede+ cells of Total Kaede + cells | 3 dpf | 30 pg vp16-hoxb5b | T-test | 0.10 | 7 | 7 |
5S | I | # Gut Restricted Phox2b + Cells | 50 hpf | 15 pg vp16-hoxb5b | T-test | 5.68E-04 | 10 | 7 |
5S | I | # Gut Restricted Phox2b + Cells | 50 hpf | 30 pg vp16-hoxb5b | Welch’s T-test | 3.98E-04 | 10 | 9 |
5S | J | Cell Fraction, POD Localized | 50 hpf | 15 pg vp16-hoxb5b | T-test | 0.25 | 10 | 7 |
5S | J | Cell Fraction, POD Localized | 50 hpf | 30 pg vp16-hoxb5b | T-test | 0.37 | 10 | 9 |
5S | J | Cell Fraction, Gut Localized | 50 hpf | 15 pg vp16-hoxb5b | T-test | 0.25 | 10 | 7 |
5S | J | Cell Fraction, Gut Localized | 50 hpf | 30 pg vp16-hoxb5b | T-test | 0.35 | 10 | 9 |
6 | H | elavl3/4+ cells | HS 14|3 dpf | hsp70:GFP-hoxb5b+ | T-test | 2.32E-04 | 6 | 11 |
6 | H | elavl3/4 + cells | HS 21|3 dpf | hsp70:GFP-hoxb5b+ | T-test | 1.68E-03 | 6 | 10 |
6 | H | elavl3/4 + cells | HS 48|3 dpf | hsp70:GFP-hoxb5b+ | T-test | 0.58 | 12 | 14 |
7 | C | # GFP + cells | 63 hpf | Enteric GFP+ | T-test | 0.26 | 6 | 6 |
7 | C | # Phox2b + cells | 63 hpf | Enteric Phox2b+ | T-test | 0.79 | 6 | 6 |
7 | D | Fraction Phox2b + copositive sox10:GFP cells | 63 hpf | 15 pg vp16-hoxb5b | T-test | 0.13 | 6 | 6 |