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Workflow. Surgical resections of previously untreated tumors were dissociated into a single cell solution and seeded together with cultured normal human fibroblasts in specialized plates. Spheroids were maintained, monitored over time, and analyzed by a variety of assays including IHC assays or end-point cell viability measurements (created with BioRender.com). IHC, immunohistochemistry; IF, immunofluorescence.
