FIG. 1.

H7-specific fliC primers as part of a multiplex VF PCR assay. VF-specific PCR products (lane 2), labeled as to gene and size (base pairs), were amplified simultaneously in a single PCR using primer pool IV, which contains eight primer pairs (two pairs for cdtB, which yield products of the same size, plus one pair each for the other six genes). The template DNA contained all seven genes of interest. Lane 1 (M),100-bp molecular weight ladder.