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. 2022 Feb 14;7:40. doi: 10.1038/s41392-021-00865-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — CircPUM1 enhances the tumorigenicity of ESCC cells in vitro and in vivo. a Fractionation of KYSE30 cells following by RT-qPCR was used to determine the localization of circPUM1. GAPDH served as the cytoplasmic expression control and NEAT1 severed as the nuclear expression control. b RT-qPCR analysis of circPUM1 expression profile in ESCC cell lines that normalized by normal esophageal epithelial cell line NE2. c RT-qPCR analysis of circPUM1 after knocking down circPUM1 in KYSE30 cell. d RT-qPCR analysis of mPUM1 after knocking down circPUM1 in KYSE30 cell. e The growth curve that monitored by the RTCA-MP system and colony formation analyses following the depletion of circPUM1 in KYSE30 cell. f The growth curve that monitored by the RTCA-MP system and colony formation analyses following the overexpression of circPUM1 in KYSE410 cell. g Macroscopic appearance of the xenograft tumors in nude mice after injection with KYSE30-sh-con (left side on both), KYSE30-sh-circPUM1-1 (right side) (up) and KYSE30-sh-circPUM1-2 (right side) (down). Xenograft tumors were isolated, KYSE30-sh-con (up on both side), KYSE30-sh-circPUM1-1 and KYSE30-sh-circPUM1-2 (down on both side). h–j Comparison of the growth of KYSE30 xenografts expressing sh-con versus sh-circPUM1 in tumor volume (h) and tumor weight (i); IHC that with Ki67 antibody staining (j), the scale bar is 200 μm. k Elevation of circPUM1 in ESCC patients was associated with worse overall survival of ESCC patients