Fig. 3.

CircPUM1 interacts with UQCRC2 in mitochondria. a Analysis pipeline was performed to identify proteins that interact with circPUM1: (1) Screening 20 Mito-proteins from three bands, of which 111 proteins in band 1, 83 proteins in band 2, 17 proteins in band 3; (2) Selecting Mito-proteins that ranked the top five in each band; (3) RNA pulldown-Western Blot and RIP-qPCR analysis confirmed the interaction of UQCRC2 and circPUM1. b Mass spectrometry assay depicted the UQCRC2 peptides pulled down by anti-sense circPUM1 probes. c Biotin-labeled sense or antisense circPUM1 probes were used for RNA-protein pull-down against KYSE30 cells lysates following western blot analysis of UQCRC2 in precipitated proteins. d RIP assay in KYSE30 cells using UQCRC2 antibody. e Diagrams showing the interaction of circPUM1 and UQCRC2. f Confocal analysis of circPUM1 (green) and UQCRC2 (blue) in KYSE30 cells, the scale bar is 10 μm. g FISH detection of circPUM1 (green) and the co-localization with mitochondria (red), the scale bar is 10 μm