Fig. 4.
CircPUM1 participates in the mitochondrial oxidative phosphorylation through interacting with UQCRC2. a RT-qPCR assay to determine the circPUM1 expression in mitochondria that isolated from KYSE30 cell after treating with 200 μM CoCl2 for 18 h. b RT-qPCR assay to determine the circPUM1 expression in mitochondria that isolated from KYSE30 cell after knocking down of circPUM1. c RT-qPCR assay to determine the circPUM1 expression in mitochondria that isolated from KYSE410 cell after overexpression of circPUM1. d Flow cytometry was performed to test hypoxic cells after knocking down circPUM1 with or without CoCl2 stimulation in KYSE30 cell. e Flow cytometry was performed to test hypoxic cells after knocking down circPUM1 in circPUM1- overexpressed KYSE30 cell. f OCR was measured by Seahorse XF assays after knocking down circPUM1 in KYSE30 cell. Basal and maximal OCR levels were at the right side. g OCR was measured by Seahorse XF assays after knocking down UQCRC2 in KYSE30 cell. Basal and maximal OCR levels were at the right side. h OCR was measured by Seahorse XF assays after overexpressing circPUM1 in both KYSE30 cell and KYSE410 cell. Basal and maximal OCR levels were at the right side. i ATP generation assay was performed to test the ATP generation ability in KYSE30 cells (knockdown of circPUM1 or UQCRC2), as well as in KYSE30 and KYSE410 (overexpression of circPUM1). j ECAR was measured by Seahorse XF assays after knocking down circPUM1 in KYSE30 cell. Basal and maximal ECAR levels were at the right side. k ECAR was measured by Seahorse XF assays after overexpressing circPUM1 in both KYSE30 cell and KYSE410 cell. Basal and maximal ECAR levels were at the right side