Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 14;7:40. doi: 10.1038/s41392-021-00865-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — CircPUM1 maintains mitochondrial homeostasis by regulating the mitochondrial complex III assembly. a Co-IP analysis for the binding affinity between UQCRC2 and UQCRC1 after knockdown of circPUM1 in KYSE30 cell. b Co-IP analysis for the binding affinity between UQCRC2 and UQCRC1 after overexpressing circPUM1 in KYSE410 cell. c, d Fluorescence microscope was used to observe the change of mitochondrial membrane potential (MMP) after knocking down circPUM1 and UQCRC2 in KYSE30 cell, the scale bar is 200 μm. e Flow cytometry was used to analyze the MMP after overexpressing circPUM1 in KYSE410 cells. f Flow cytometry was used to analyze the ROS generation after knocking down circPUM1 and UQCRC2 in KYSE30 cells. g Flow cytometry was used to analyze the ROS generation after overexpressing circPUM1 in KYSE410 cells. h Flow cytometry was used to analyze the MitoSox generation after knocking down circPUM1 and UQCRC2 in KYSE30 cells. i Flow cytometry was used to analyze the MitoSox generation after overexpressing circPUM1 in KYSE410 cells. j Super Resolution Microscope was used to observe mitochondrial morphology after knocking down circPUM1 in KYSE30 cell