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. 2022 Feb 14;7:40. doi: 10.1038/s41392-021-00865-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — CircPUM1 could inhibit the pyroptosis in ESCC cells. a Phase-contrast cell images (arrows, pyroptotic cells) after knocking down circPUM1 in KYSE30 cell, the scale bar is 100 μm. b Western blot analysis of knocking down circPUM1-induced GSDME cleavage but not GSDMD cleavage in KYSE30 cell. c GSDME expression profile in ESCC cell lines examined by western blot. d Western blot analysis of caspase3 and cleaved caspase3 after knocking down circPUM1 in KYSE30 cell. e Western blot analysis of pyroptosis-related proteins in KYSE30 cell after knocking down circPUM1. f Western blot analysis of the regulatory function of AMPK to the cleavage of GSDME following adding 2 μM AMPK inhibitor (Dorsomorphin dihydrochloride) in circPUM1 knockdown cell in KYSE30 cell and 200 μM AMPK activator (AICAR) in circPUM1 overexpressed cell in KYSE410 cell. g LDH release analysis after knockdown of circPUM1 and UQCRC2. h Immunofluorescence (IF) was performed to evaluate E-cadherin staining on KYSE30 cell after knocking down circPUM1, the scale bar is 30 μm