Figure 4.

hOTC expression in the liver at different time points by immunohistochemistry

hOTC transgene expression by hepatocytes was analyzed in liver sections of individual cynomolgus macaques by performing immunohistochemistry (IHC) at day 28, day 84, and day 140. (A) Representative IHC images (20×) are shown for one animal from each group. Non-study control and OTCD patient control samples are also shown. Scale bar indicates 100 μm. (B–E) hOTC expression was analyzed using Visiopharm software. Proportion of OTC-positive area is shown by day (B) and for individual groups (C–E). For day 28 and day 84, data are represented as individual values for each animal with mean ± SEM. For day 140, data are represented as the mean of values from four lobes from each animal with mean ± SEM. OTCD, ornithine transcarbamylase deficiency; RE, right edge. See also Figure S7.