FIGURE 5.

Recovery of glutamate uptake in striatal astrocytes. (A) Measurement of glutamate uptake by sodium imaging with SBFI, in the presence of CBX (100 μM), DNQX (10 μM), and MK801 (1 μM). Traces recorded in the absence and presence of TFB-TBOA (2 μM). The amplitude of this differential response was expressed as ΔF/F during the last 15 s of L-Asp application. F is the mean fluorescence at rest, before drug application. (B) Tested animal groups. (C) Averaged traces of SBFI fluorescence. Results from mRuby-positive astrocytes only. (D) Quantification of the L-aspartate-induced sodium elevation. Two-level (“nested”) statistics (animal level, cell level, and post hoc comparison between groups). (E) The applied SBFI loading protocol was selective for astrocytes. View field with 3 astrocytes, where one is transduced and two are not. (F) Traces from mRuby+ as opposed to mRuby– in three test groups. Averaged traces from one animal per group. (G) Comparison of results obtained in the different groups from mRuby+ vs. mRuby– astrocytes. *p < 0.05, **p < 0.01.