TABLE 2.
Summary of experiments with functional testing.
| mHTT-sensitive functional indicator | WT-CTRL |
HET-CTRL |
HET-EAAT2-S506X |
Statistics |
Post hoc MC HET-CTRL vs. HET-S506X |
|||||||||||||
| Mean | SE | N-c/t | N-a | Mean | SE | N-c/t | N-a | Mean | SE | N-c/t | N-a | Test | F (DFn, Dfd) | P | Test | P | Hedges’ G | |
| #CAG repeats | nd | 186.1 | 1.2 | 22 | 185.6 | 1.7 | 15 | t-test | ns | |||||||||
| Body weight at sacrifice | 31.28 | 0.86 | 21 | 25.35 | 0.77 | 22 | 26.41 | 1.08 | 15 | ANOVA | 0.29 (2, 55) | <0.0001 | Tukey | ns | ||||
| OF total distance traveled (m/5 min) | 18.72 | 0.93 | 14 | 12.99 | 1.77 | 15 | 19.16 | 1.80 | 21 | ANOVA | 4.09 (2, 47) | 0.0230 | Tukey | 0.027 | −0.81 | |||
| OF #starts/stops (in 5 min) | 61.07 | 4.78 | 14 | 36.80 | 6.23 | 15 | 58.67 | 6.66 | 21 | Kruskal–Wallis | 4.17 (2, 47) | 0.0216 | B-K-Y | 0.040 | −0.78 | |||
| Step-over latency (s) | 56.7 | 10.00 | 18 | 179.1 | 46.6 | 19 | 51.6 | 9.8 | 14 | Kruskal–Wallis | 7.37 (2, 47) | 0.0251 | B-K-Y | 0.041 | 0.79 | |||
| Maximal/mean rest radius | 2.53 | 0.04 | 14 | 2.81 | 0.11 | 15 | 2.58 | 0.21 | 21 | ANOVA | 4.64 (2, 47) | 0.01 | Tukey | 0.038 | 0.75 | |||
| Mean running velocity (mm/s) | 99.53 | 5.14 | 14 | 81.57 | 6.04 | 15 | 109.45 | 8.06 | 21 | ANOVA | 4.15 (2, 26) | 0.0220 | Tukey | 0.017 | −0.87 | |||
| Mean velocity at rest (mm/s) | 37.39 | 1.78 | 14 | 26.48 | 3.69 | 15 | 36.91 | 3.12 | 21 | ANOVA | 3.72 (2, 47) | 0.0318 | Tukey | 0.048 | −0.70 | |||
| Open field center time (%) | 31.48 | 4.32 | 14 | 14.98 | 3.67 | 15 | 16.57 | 2.47 | 21 | ANOVA | 6.59 (2, 47) | 0.0030 | Tukey | ns | ||||
| Syn Glu transient spread (μ m) | 1.23 | 0.05 | 48 | 14 | 1.45 | 0.08 | 46 | 21 | 0.94 | 0.08 | 29 | 8 | Nested ANOVA | 6.282 (2, 40) | 0.0042 | B-K-Y | 0.0011 | 1.03 |
| Syn Glu transient(max) TauD (ms) | 3.03 | 0.28 | 48 | 14 | 8.21 | 1.39 | 46 | 21 | 2.57 | 0.31 | 28 | 8 | Nested ANOVA | 4.528 (2, 40) | 0.0169 | B-K-Y | 0.012 | 0.76 |
| Syn Glu transient(max) amp (DF/F%) | 74.85 | 9.21 | 48 | 14 | 64.20 | 6.77 | 46 | 21 | 53.5 | 8.3 | 28 | 8 | Nested ANOVA | 0.31 (2, 40) | 0.4751 | B-K-Y | ns | 0.24 |
| Syn EAAT2 IF total (a.u.)* | 5230 | 168.7 | 12/360 | 2 | 3663 | 81.42 | 12/360 | 2 | 4638 | 205.8 | 12/360 | 2 | Nested ANOVA | 12.25 (3,4) | 0.0175 | Dunnett | 0.048 | −1.87 |
| SPN input resistance (MOhm) | 114 | 10.3 | 18 | 8 | 165 | 17.0 | 14 | 8 | 127 | 13.5 | 8 | 5 | Nested ANOVA | 4.05 (2, 37) | 0.0256 | B-K-Y | 0.104 | 0.64 |
| ITonic(GABA) amp (pA) | 11.33 | 2.37 | 13 | 6 | 2.99 | 1.03 | 14 | 8 | 5.78 | 3.37 | 7 | 5 | Nested ANOVA | 6.19 (2, 16) | 0.0102 | B-K-Y | ns | |
*Test includes 4KR group.
MC, multiple comparison test; BKY, Benjamini, Krieger, Yekutieli test. In bold: Indicators with sensitivity to X-effect.