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. 2022 Jan 31;10:829868. doi: 10.3389/fbioe.2022.829868

FIGURE 2.

FIGURE 2

HUCMSCs can regulate oxidative stress damage of HUVECs through exosomes. (A) Western blotting results of NOX1 and NOX4 in HUVECs following different treatments. (B) Reduction of ROS in HUVECs assessed through flow cytometry with the DCFH-DA probe following different treatments. (C) Quantitation of ROS depletion measured by the intensity of fluorescence of HUVECs. (D) A tube formation assay was performed to visualize the cell capillary network formation of HUVECs. Scale bar: 100 μm. (E, F) Quantitative analysis of the tube formation of HUVECs in the three groups. (G) RT-qPCR results of cyclin D1 and cyclin D3 expressions in the HUVECs following different treatments. (H–J) RT-qPCR results of IL-1β, IL-6, and TNF-α expressions in the HUVECs following different treatments. Differences were measured by one-way ANOVA followed by a Tukey post hoc test for pairwise comparison. Data presented as means ± SD. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05.