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. 2022 Jan 31;10:829868. doi: 10.3389/fbioe.2022.829868

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Copyright © 2022 Yan, Xv, Lin, Endo, Xue, Hu, Hu, Chen, Cao, Zhou, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of HUC-Exos (exosomes secreted by HUCMSCs). (A) Representative picture of the ultrastructure of exosomes observed by transmission electron microscopy (TEM). (B) The average particle size distribution of normal-Exos and diabetes-Exos was measured by dynamic light scattering (DLS). (C) The marker protein levels of CD9, CD81, and TSG101 in the isolated exosomes were detected with Western blotting. Differences were measured by one-way ANOVA followed by a Tukey post hoc test for pairwise comparison. Data presented as means ± SD. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05.