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. 2022 Jan 31;10:829868. doi: 10.3389/fbioe.2022.829868

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Copyright © 2022 Yan, Xv, Lin, Endo, Xue, Hu, Hu, Chen, Cao, Zhou, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HUC-Exos enhanced angiogenesis in vivo. Mice were treated with PBS, 50 μg/ml of UMSCs-Exos, and 100 μg/ml of UMSCs-Exos (n = 10). (A) The perfusion of the wound area in the three groups was assessed using a small-animal Doppler analysis. Scale bar: 100 μm. (B) Quantitative perfusion intensity of the wound area in the three groups. (C) Immunofluorescence assay with marker CD31 (+), n = 10. Scale bar: 100 μm. (D) Fluorescence quantification of the wound length at day 14. Scale bar: 50 μm. Differences were measured by one-way ANOVA followed by a Tukey post hoc test for pairwise comparison. Data presented as means ± SD. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05.