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. 2022 Jan 31;8:826687. doi: 10.3389/fcvm.2021.826687

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Copyright © 2022 Quiroz, Valencia, Shao, Li, Ortiz, Parikh, Lassance-Soares, Vazquez-Padron, Liu and Velazquez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of Mesenchymal Stem Cells (MSC). (A) MSC demonstrated fibroblast-like morphology in the growth medium and were adherent to the cell culture dish. (B) Flow cytometry revealed the native MSC had expression of cell-surface molecules CD44, CD73, and CD105. (C) After cells were transduced with either GFP/AAV or E-selectin-ires-eGFP/AAV, successful cell transduction is demonstrated by expression of GFP under confocal microscopy. (D) Flow-cytometry demonstrates a lack of E-selectin expression in untransduced MSC (top) and a gain of E-selectin expression after transduction has been performed (bottom).