Figure 6.

In vivo relative gene expression changes in the ischemic thigh tissue of mice treated with MSC^{Eselectin−GFP} vs. MSC^GFP. (A) Expression of several chemokine/cytokine mRNA was significantly higher in the ischemic thigh muscle treated with MSC^{Eselectin−GFP} vs. MSC^GFP measured by quantitative PCRArray by post-operative day 21. However, tumor necrosis factor (TNF) was significantly downregulated. Equal portions of RNA from three mice in each treatment group were utilized to perform reverse transcription reaction. Each experiment was repeated three times and data are presented as fold-change of treatment/control (*P < 0.05 via Student's T-test). (B) Table listing full name of upregulated genes with the † mark indicating the genes also upregulated in-vitro within the injected supercharged MSC^{E−selectin−GFP} vs. the control MSC^GFP.