Figure 7.

Determining the fate of MSC injected as treatment for hindlimb ischemia 3-weeks after injection into thigh muscle. (A). Representative images of immunohistochemistry co-staining with DAPI and GFP (AF-594), demonstrating that a few of the injected MSC survive at 3-weeks (POD 21). (B) Quantitative analysis of mean fluorescent intensity (MFI) in ischemic thigh muscle tissue sections stained for GFP demonstrates significant increased MFI between MSC^{E−selectin−GFP}, MSC^GFP, and Vehicle (n = 30 sections/group, **P < 0.001, *P < 0.01, n.s. = not significant via ANOVA with post-hoc Tukey Simultaneous Tests for Differences of Means) (C). Representative PCR gel electrophoresis of mice lung and liver tissue demonstrating lack of GFP expression (positive control located at 282BP) and positive β-actin for MSC^{E−selectin−GFP}, MSC^GFP, and Vehicle (at 218BP).