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. 2022 Jan 31;12:678025. doi: 10.3389/fonc.2022.678025

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Copyright © 2022 Qiu, Wu, Shi, Liu, Li, Wu and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ASF1B could promote proliferation and inhibit apoptosis of male TC cells and accelerate cell cycle. (A) The knockdown efficiency of ASFB1 in KTC-1 cells was measured by real-time PCR. sh-ASF1B-3 (sh3) showed the best silencing effect and was used for the subsequent experiments, *P < 0.05. (B) Cell proliferation were detected by CCK-8 assays in KTC-1 cells, *P < 0.05. Detection of cell cycle (C) and cell apoptosis (D) in KTC-1 cells after transfection with sh-NC or sh-ASF1B by flow cytometry, respectively, *P < 0.05.