Table 2.
Primer sequences used for real-time PCR.
| Gene | Primer sequence (5’ –3’) | Product length (bp) | GeneBank accession No. |
|---|---|---|---|
| TNF-α | F: CGTGAAGCTGAAAGACAACCAG | 121 | NM_214022.1 |
| R: GATGGTGTGAGTGAGGAAAACG | |||
| IL-1β | F: CAGCTGCAAATCTCTCACCA | 112 | NM_214055.1 |
| R: TCTTCATCGGCTTCTCCACT | |||
| IL-6 | F: TTCACCTCTCCGGACAAAAC | 122 | NM_001252429.1 |
| R: TCTGCCAGTACCTCCTTGCT | |||
| IL-8 | F: AGTTTTCCTGCTTTCTGCAGCT R: TGGCATCGAAGTTCTGCACT | 72 | NM_213867.1 |
| IL-10 | F: CCTGGAAGACGTAATGCCGA R: CACGGCCTTGCTCTTGTTTT | 148 | NM_214041.1 |
| RIG-I | F: AGAGCAGCGGCGGAATC | 82 | NM_213804.2 |
| R: GGCCATGTAGCTCAGGATGAA | |||
| MDA5 | F: TCCGGGAAACAGGCAACTC | 75 | NM_001100194.1 |
| R: CAAAGGATGGAGAGGGCAAGT | |||
| MAVS | F: TGGGTACAGTCCTTCATCGG R: GGGTAACTTGGCTCATCCTCT | 116 | NM_020746.5 |
| TLR2 | F: TCACTTGTCTAACTTATCATCCTCTTG | 162 | XM_005653576.3 |
| R: TCAGCGAAGGTGTCATTATTGC | |||
| TLR3 | F: TGGAAAAAGGAATGGCCAGC R: ACAAGGCAAACTCCTGCTCA | 265 | NM_001042467.3 |
| TLR4 | F: TTACAGAAGCTGGTTGCCGT R: TCCAGGTTGGGCAGGTTAGA | 152 | NM-001293316.1 |
| TLR7 | F: CAATGGTCCCTGAGCGTTTG R: AGCCTGGTTGAAGACAGCAG | 126 | NM_016562 |
| TLR9 | F: CACGACAGCCGAATAGCAC | 121 | NM_213958.1 |
| R: GGGAACAGGGAGCAGAGC | |||
| MyD88 | F: GTGCCGTCGGATGGTAGTG | 65 | NM001099923 |
| R: TCTGGAAGTCACATTCCTTGCTT | |||
| TRIF | F: CAAGTGGAGGAAGGAACAGG R: CAACTGCGTCTGGTAGGACA | 139 | XM_003362039.1 |
| TRAF6 | F: CAAGAGAATACCCAGTCGCACA R: ATCCGAGACAAAGGGGAAGAA | 122 | NM-001105286.1 |
| β-actin | F: GGATGACGATATTGCTGCGC | 190 | XM_003124280.5 |
| R: GATGCCTCTCTTGCTCTGGG |
TNF-α, tumor necrosis factor-α; IL, interleukin; RIG-I, retinoic acid-inducible gene I; MDA5, melanoma differentiation associated gene 5; MAVS, mitochondrial antiviral signaling protein; TLR, toll-like receptor; MYD88, myeloid differentiation factor 88; TRIF, TIR domain-containing adaptor inducing interferon β; TRAF6, TNF receptor-associated factor 6