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. 2022 Jan 31;13:779574. doi: 10.3389/fimmu.2022.779574

Figure 2.

Figure 2

The migration and LIF production of the human mast cell line LAD2 are increased when co-cultured with decidualized T HESCs compared with non-decidualized T HESCs. (A) Two-chamber assay (n = 3). The upper chambers containing LAD2 cells were inserted into the lower chambers, which contained culture medium only (“none”), or decidualized or non-decidualized T HESCs cells. After an 8-hour incubation, the cells migrating into the lower chambers were counted. *P < 0.05 compared with none and **P < 0.05 compared with non-decidualized T HESCs in the lower chamber. (B) LIF ELISA (n = 5). LAD2 cells were cultured in cytokine-free medium only (“none”), medium with SCF (“none + SCF”), and cytokine-free medium with non-decidualized or decidualized T HESCs for 8 (h) The supernatants were collected and used in ELISA kits. *P < 0.05 compared with none and **P < 0.05 compared with none + SCF.