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PEITC inhibited pyroptosis in AML12 cells treated with LPS and ATP in vitro. (A) Representative electron micrographs of cell morphology from each group. Scale bar, 5 µm. (B, C) Immunoblot and statistical analysis of NLRP3, pro-CASP1, cleaved CASP1, GSDMD, GSDMD-N, and β-actin. (D) Immunofluorescence staining of GSDMD. Scale bar, 50 µm. Data are presented as means ± SEM. ##p < 0.01, ###p < 0.001 vs. blank, *p < 0.05, **p < 0.01 ***p < 0.001 vs. LPS+ATP control.
