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. 2022 Feb 14;380(2220):20210109. doi: 10.1098/rsta.2021.0109

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© 2022 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — Pupils and Ewald spheres for light sheet structured illumination microscopy approaches. (a) Single pupil and illumination pattern for lattice light sheet microscopy. As desired, the Fourier intensity distribution consists of harmonic functions laterally. (b) Opposed pupils using counterpropagating light sheets for SIM. Here, the components of the Fourier intensity distribution are stretched out along the pattern direction, precluding conventional reconstructions in which harmonic components are assumed. (c) A potential method for producing lattice-like illumination patterns at spatial frequencies beyond that achievable with one objective. Here, C-shaped illumination profiles in the auxilliary objectives are used to introduce the off-centre Ewald sphere components, ensuring a harmonic Fourier intensity distribution. (Online version in colour.)