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. 2022 Jan 31;8:785160. doi: 10.3389/fmolb.2021.785160

FIGURE 1.

FIGURE 1

CRISPR-dCas13 enables visualization of TERRA in live cells. (A) Overview of CRISPR-dCas13-mediated TERRA labeling. (B) Representative images of dCas13b-EGFP with three different guide RNAs for TERRA and guide for non-target control RNA. Arrows indicate dCas13b-EGFP labeled TERRA. (C) Quantification of total foci per cell indicated by dCas13 with different guide RNAs (mean ± SEM, unpaired t-test). N ≥ 100 for each group. ***p < 0.001. (D) RNA FISH shows colocalization of dCas13b-EGFP with TERRA foci (red). DAPI detects nuclear DNA.