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. 2022 Jan 31;8:785160. doi: 10.3389/fmolb.2021.785160

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Copyright © 2022 Xu, Chigumira, Chen, Tones, Zhao, Dahl, Chenoweth and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SunTag increases TERRA labeling efficiency. (A) Plasmid map of dCas13b tagged with SunTag, including five repeats of GCN4, single-chain variable fragment antibody (scFV) followed by superfolded GFP (sfGFP). (B) TERRA RNA FISH shows co-localization of dCas13b-SunTag-sfGFP with TERRA FISH probe (orange). (C) Quantification for percentage of TERRA FISH foci labeled by dCas13b-EGFP and dCas13b-SunTag-sfGFP (mean ± SEM, unpaired t-test). N ≥ 80 for each group. ***p < 0.001. (D) Quantification for percentage of dCas13b-EGFP and dCas13b-SunTag-sfGFP labeled by TERRA FISH (mean ± SEM). N ≥ 30 for each group.