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. 2022 Jan 31;12:742704. doi: 10.3389/fgene.2021.742704

FIGURE 1.

FIGURE 1

Alteration of MAPK/ERK signaling pathway in offspring heart of overfed ewes at two gestational and one early postnatal time point. (A–G). Relative expression and phosphorylation level of hallmark proteins in fetal heart at mid-gestation day 90; (H–N). Relative expression and phosphorylation level of hallmark proteins in fetal heart at late-gestation day 135; (O–U). Relative expression and phosphorylation level of hallmark proteins in neonatal heart at day 1 after birth; GAPDH, Protein loading control. CON, control, MO, maternal obesity, M, male, and F, female. Mean ± SEM (n = 5); *p < 0.05, **p < 0.01.