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. 2022 Jan 31;12:742704. doi: 10.3389/fgene.2021.742704

FIGURE 5.

FIGURE 5

Alteration of inflammatory cytokine NF-κB and TNFα in offspring heart of overfed ewes at two gestational and one early postnatal time point. (A–C). Relative expression level of inflammatory cytokine in fetal heart at mid-gestation day 90; (D–F). Relative expression level of inflammatory cytokine in fetal heart at late-gestation day 135; (G–I). Relative expression level of inflammatory cytokine in neonatal heart at day 1 after birth; GAPDH, Protein loading control. CON, control, MO, maternal obesity, M, male, and F, female. Mean ± SEM (n = 5); *p < 0.05, **p < 0.01.