TABLE 4.

Enzymatic conversion of chitosan to COSs.

Enzyme	Amount of enzyme	Chitosan	Reaction time (h)	Hydrolysis rates (%)	DP of major products	References
ShCsn46	10 U/ml (about 1%)	4% with 95% DDA	0.33	91.2	2–6	This study
Chitosanase from A. fumigatus CJ22-326	30 U/ml	2% with 95% DDA	8	97.3	2–6	Zhou et al., 2020
PbCsn8	5 U/ml	5 with 85% DDA	4	79.3	2–4	Jiang et al., 2021
BaCsn46A	3 U/ml	3 with 95% DDA	3	86.9	2–6	Qin et al., 2018
Csn-SH	20 U/ml	4% with 85% DDA	0.67	80.6	2–6	Cui et al., 2021
CsnBm	9 U/ml	4% with 90% DDA	0.25	92.3	2–6	Wang J. R. et al., 2021
Commercial pectinase	10%	1% with 88% DDA	24	17.0	6–11	Cabrera and Van Cutsem, 2005
Commercial lipase	0.5%	0.5% with 83% DDA	6	58.2	1–6	Lee et al., 2008
Commercial papain	0.3%	1% with 87% DDA	24	11.1	3–7	Lin et al., 2002
Commercial cellulase	0.25%	0.5% with 87% DDA	24	40.0	2–6	Tegl et al., 2016