FIGURE 5.

Mechanical tension-iniated autophagy promotes FGF23 secretion. (A) The mRNA expression level of FGF23 MLO-Y4 after mechanical tension was measured by RT-PCR. GAPDH was used for normalization. (B) The expression of soluble FGF23 in the supernatant of MLO-Y4 after mechanical tension was detected by ELISA. (C) Western blot analysis of total protein expression of FGF23. GAPDH was used for normalization. All cell experiments were repeated 3 time and each time they were performed in at least 3 replicate wells. All data were showed as means ± SD. *p ˂ 0.05 vs. control.