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(a) ARS staining of SHEDs cultured on different substrates with PM/OM medium for 7 days. Scale bars = 100 μm (b) DMP-1 and (c) DSPP genes expression detected by RT-PCR, (d) DMP-1 and (e) DSPP production detected by CLSM images. Green fluorescence indicates DMP-1 or DSPP, red fluorescence indicates actin fibers, and blue fluorescence indicates nuclei. Scale bars = 50 μm.
