RT-PCR analysis for VEGF (a) and VEGF-R2 (b) genes expression in SHEDs. (c, d) HUVECs migration cultured in conditioned medium (SHEDs, SHEDs/CMS and SHED/SMS) and corresponding quantitative analysis of migration distance. (e, f) In vitro tubule formation of HUVECs induced by conditioned medium (SHEDs, SHEDs/CMS and SHED/SMS) and the corresponding quantitative analysis of total tubules length. (g. h) CAM assay for neovascular formation evaluation and corresponding quantitative analysis of total vessels length. Samples were stimulated with SHEDs, SHEDs/CMS or SHED/SMS conditioned medium. Scale bars = 400 μm.