Table II.

Validation Parameters for NOMG, OMG, NOM, OM, 6α-Oxycodol, 6β-Oxycodol, NOC and OC

			Interday precision (%CV)			Intraday precision (%CV)			Bias (%)				Process efficiency (%) Analyte (d3)
Analyte	LLOQ (μg/mL)	ULOQ (μg/mL)	Lowa	Midb	Highc	Lowa	Midb	Highc	Lowa	Midb	Highc	Dilution integrity (%)	Lowd	Highe
NOMG	0.05	10	10.5	3.6	3.8	12.9	4.3	4.4	−2.1	−5.1	−2.0	111	81	71
OMG	0.05	10	5.5	2.9	3.4	6.7	3.6	3.9	2.4	−10.2	−3.7	100	98 (105)	91 (92)
NOM	0.015	10	8.5	2.8	4.6	10.4	3.4	5.2	−0.4	−1.3	−5.5	101	86	105
OM	0.015	10	10.6	3.0	3.5	13.0	3.6	4.0	−10.9	−2.0	−6.2	98	93 (91)	96 (94)
αOCL	0.015	10	6.2	2.1	2.6	5.0	1.8	2.9	−4.4	−3.2	−9.8	101	109	114
βOCL	0.015	10	8.8	1.8	2.8	10.8	2.2	3.2	−5.3	−3.4	−4.6	98	102	109
NOC	0.015	10	5.5	2.0	2.9	6.8	2.4	3.3	−10.2	−5.5	−11.8	100	113 (119)	118 (115)
OC	0.015	10	7.4	2.2	2.2	9.0	2.7	2.6	−10.0	−7.3	−12.2	103	118 (118)	116 (114)

^aLow concentration: 0.03 μg/mL for NOM, OM, αOCL, βOCL, NOC and OC, 0.1 μg/mL for NOMG and OMG.

^bMiddle concentration: 4 μg/mL for all analytes.

^cHigh concentration: 8 μg/mL for all analytes.

^dLow concentration was 0.2 μg/mL for all analytes.

^eHigh concentration was 2 μg/mL for all analytes.