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. 2022 Feb 8;76:103867. doi: 10.1016/j.ebiom.2022.103867

Figure 1.

Figure1

Schematics of the PCT/CRP duplex lateral flow assay (LFA) and signal intensity quantification protocol. (a) Schematics of the PCT/CRP duplex LFA. A serum sample containing PCT and CRP antigens is added to the sample pad (Sizes of PCT and CRP antigens not to scale). The conjugate pad contains both PCT and CRP detection antibodies pre-conjugated with EuNPs. PCT and CRP antigens will bind to EuNP-conjugated PCT and CRP detection antibodies, respectively. As the sample flows through the nitrocellulose pad from left to right, the europium-conjugated CRP detection antibodies and PCT detection antibodies are captured by the first line (CRP line) and the second line (PCT line), respectively. Unbound conjugates are captured by the third line (control line). (b) Image processing protocol to quantify signal intensities of test and control lines. The three lines shown in the figure represent the CRP test line, the PCT test line, and the control line, from bottom to top. The bottom of the region of interest represents pixel position at 0, and its top represents pixel position at 1499. The width of the region of interest is 500.