Skip to main content
. 2022 Feb 5;8(2):e08904. doi: 10.1016/j.heliyon.2022.e08904

Figure 2.

Figure 2

MPPs induced by chronic IFN- I exposure do not show active proliferation or differentiation in dental pulp. (A) Experimental design and time-course for acute and chronic poly(I:C) treatment. (B) Percentages of CD34-KSLs, multipotent progenitor cells (MPPs), and lymphoid-primed multipotent progenitor cells (LMPPs) in BM, DP, and PB of poly(I:C)-treated mice and non-treated mice at 24 h after chronic poly(I:C) treatment. Bars represent the mean ± SEM. Significance was calculated using two-way analysis of variance. ∗ denotes P < 0.05, ∗∗ denotes P < 0.01, ∗∗∗∗ denotes P < 0.0001, and n.s. denotes not significant. (6–8 mice/group, n = 16). (C) CFU assay using dental pulp cells of chronic poly(I:C)-treated and non-treated mice. The left two graphs show the number and size of colonies on day 12. The right two graphs show the number and percentage of burst-forming unit-erythroid (BFU-E); colony-forming unit-granulocyte, macrophage (CFU-GM); colony-forming unit-granulocyte (CFU-G); colony-forming unit-macrophage (CFU-M); colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM); and colony-forming unit-granulocyte, macrophage, megakaryocyte (CFU-GMM). Bars represent the mean ± SEM. Significance was calculated using the unpaired t-test and two-way analysis of variance. ∗P < 0.05, ∗∗∗ denotes P < 0.001, ∗∗∗∗ denotes P < 0.0001, and n.s. denotes not significant. (n = 18).