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. 2022 Feb 5;8(2):e08904. doi: 10.1016/j.heliyon.2022.e08904

Figure 3.

Figure 3

Acute IFN-I exposure induces myeloid progenitor cells in dental pulp. (A) Percentages of CD34-KSL, MPPs, and LMPPs in BM, DP, and PB of poly(I:C)-treated mice and non-treated mice at 24 h after acute poly(I:C) treatment. Bars represent the mean ± SEM. Significance was calculated using two-way analysis of variance. ∗ denotes P < 0.05, ∗∗∗ denotes P < 0.001, ∗∗∗∗ denotes P < 0.0001, and n.s. denotes not significant (6–12 mice/group, n = 20). (B) CFU assay using dental pulp cells of acute poly(I:C)-treated and non-treated mice. The left two graphs show the number and size of colonies on day 12. The right two graphs show the number and percentage of each type of colony. Bars represent the mean ± SEM. Significance was calculated using the unpaired t-test and two-way analysis of variance. ∗ denotes P < 0.05, ∗∗ denotes P < 0.01, ∗∗∗∗ denotes P < 0.0001, and n.s. denotes not significant (n = 12). (C) Representative image of a colony (left) (×4), (middle, right) (×20) and representative image of chromatic figures of CFU-M (×20) and CFU-GM (×20). The cells were round and their sizes varied according to the cell type and the degree of maturation.