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. 2022 Jan 19;33:100412. doi: 10.1016/j.jbo.2022.100412

Fig. 1.

Fig. 1

MiR-451a inhibits the malignant progression of osteosarcoma in vitro. (A) Q-PCR detection of miR-451a expression level in human osteosarcoma cell lines. (B-D) CCK-8, colony formation and EdU assays were performed to assess the effects of miR-451a on the proliferative ability of transfected osteosarcoma cells (HOS and 143B). (E) TUNEL assay was conducted to evaluate the apoptosis of transfected osteosarcoma cells. (F-G) Cell migration was determined by Transwell migration and wound healing assays. (H) Representative images of osteosarcoma cell morphology. (I) The protein levels of EMT-related markers (ZO-1, E-cadherin, N-cadherin and Vimentin) in transfected HOS and 143B cells with miR-451a mimics or miR-451a inhibitor were detected. *P < 0.05, **P < 0.01.